The purpose of the in vitro Chromosome Aberration (CAB) Assay is to evaluate a compound, or its metabolites, for the ability to induce chromosome aberrations in cultured mammalian somatic cells. The CAB Assay is an in vitro mammalian cell genotoxicity test, which evaluates the carcinogenic potential of a compound.
It is designed to evaluate the effect of the parent chemical, as well as its metabolites, by the addition of a liver enzyme mixture. Mitotic index, or cytotoxicity, is used to select cultures which are stressed by the presence of the compound prior to scoring for chromosome aberrations. Chinese Hamster Ovary (CHO) cells are used as the cell line. The cell growth is arrested during metaphase to allow the chromosome evaluation. This GLP assay uses three study arms, 4 hr plus metabolic activation, and 4 and 24 hr without activation.
This test is one of the GLP tests accepted by regulatory agencies for IND and NDA approval.
To learn more about the guidelines MicaGenix follows for this assay, visit http://213.253.134.43/oecd/pdfs/browseit/9747301E.PDF
